A Transient RNA Interference AssaySystem JSsw Using Arabidopsis
نویسنده
چکیده
RNA interference (RNAi) is the phenomenon that double-stranded RNA (dsRNA) induces post-transcriptional sequence-specific gene silencing in eukaryote."2] During the process of RNAi, long dsRNA is cleaved into -21 nt small interfering RNAs (siRNAs), and the siRNAs then guide sequencespecific mRNA degradation. Since the discovery in Caenorhabditis etegans,3] RNAi has been shown to eccur in many eukaryotes, including mammals and
منابع مشابه
A transient RNA interference assay system using Arabidopsis protoplasts.
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Intr...
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RNA interference is a powerful technique for suppressing gene functions in many eukaryotes including plants. Here we show that introduction of double-stranded RNA into Arabidopsis protoplasts leads to marked silencing of endogenous genes, as observed previously for transgenes [Biosci. Biotechnol. Biochem., 67, 2674-2677 (2003)]. This simple system should be useful for functional analysis of gen...
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